Library Detail Page for Petunia-C2H4

Short Name:Petunia-C2H4
Organism:Petunia hybrida
Library Name: Petunia Floral Post Ethylene
Total Sequences: 2452 sequences from 3072 clones
Average Sequence Length:462.7 (Standard deviation 114.6)
Type:cDNA Library
Tissue:all floral organs
Development Stage:anthesis
Treatment Conditions:Flowers at anthesis stage were excised from plants grown in standard greenhouses and treated with 2ppm exogenous ethylene. Entire flowers were collected at 0, 5, 10, 15, 24, and 36 hours after treatment. Total RNA was extracted from each sample, and 100 micrograms of each sample was combined for subsequent poly A+ mRNA selection and cDNA synthesis.
Cloning Host:lambda ZAPII unidirectional
Cloning Kit: Stratagene
Comments
AuthorsKenichi Shibuya, Beverly Underwood, Holly Loucas, William Farmerie, Michelle Jones, David Clark*
Contact InformationDavid G. Clark\ University of Florida\ Environmental Horticulture Dept.\ 1545 Fifield Hall\ Gainesville, FL 32611-0670\ ph: 352-392-1831 x370\ FAX: 352-392-3870\ e-mail: geranium@ufl.edu\ http://hort.ifas.ufl.edu/floriculture/floriculture_bio.htm