Library Detail Page for PPI

Short Name:PPI
Organism:Solanum tuberosum
Library Name: P. infestans challenged Leaf (Incompatible)
Total Sequences: 4918 sequences from 5434 clones
Average Sequence Length:612.0 (Standard deviation 120.0)
Type:cDNA Library
Tissue:leaf
Development Stage:6-week old leaf
Treatment Conditions:Whole plants were challenged with 450,000 sporangia/ml P.infestans US-1 (US 940501) in Biotron (Madison, Wisconsin). Incompatible pathogen interaction, plants did not show HR.
Cloning Host:E.coli SOLR
Cloning Kit:
CommentsLeaf tissue was collected at 1, 2, 5, 12, and 24 hours post challenge and frozen in liquid nitrogen immediately upon removal. Kennebec plants showed no signs of HR. Katahdin plants (susceptible to P.infestans US-1) were used as controls and showed infection. See PPC library for compatible pathogen interaction.
AuthorsZhang, P., Hernandez, M., Tornqvist, C-E., Wirtz, U., Loukoianov, A., Rangel, P., Cho, J., Chiemingo, A., Bougri, O., Buell, C.R., Ronning, C.M., Baker, B.
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