Library Detail Page for CSCH

Short Name:CSCH
Organism:Solanum tuberosum
Library Name: Common Scab-Challenged Tubers
Total Sequences: 4134 sequences from 5038 clones
Average Sequence Length:684.1 (Standard deviation 130.8)
Type:cDNA Library
Tissue:Tubers
Development Stage:
Treatment Conditions:Tubers from pathogen-free Solanum tuberosum var. Shepody, clone 1756, were inoculated with Streptomyces scabei EF-35, by applying 1 ml of a spore stock (OD650 = 0.7). Tubers were placed in trays of sand and watered from the bottom for the remainder of the experiment. Inoculated tubers were sampled at 2 days, 1 week, 2 weeks and 4 weeks post-infection. Harvested tubers were washed in distilled\ water, blotted dry, cut into small pieces and frozen in liquid nitrogen. RNA was isolated from each sample collection, pooled and used for cDNA library construction.
Cloning Host:XL10-Gold
Cloning Kit: Stratagene pBluescript II XR cDNA Library Construction Kit
Comments
AuthorsFlinn, B., Rothwell, C., Sardana, R., Griffiths, R.,\ Lague, M., De Koeyer, D., Audy, P., Goyer, C., Li, X-Q., Wang-Pruski, G., Regan, S.\
Contact InformationBarry Flinn\ bflinn@bioatlantech.nb.ca\ The Canadian Potato Genome Project - BioAtlantech Unit\ 921 College Hill Rd, Fredericton, ON, E3B 6Z9, CANADA\